SNAP-annotated 5mC-DNA-protein interactions: PDB entry 8xqc

PDB-id

8xqc; DSSR-derived features in text and JSON formats; DNAproDB

Class

transferase

Method

cryo-EM (3.25 Å)

Summary

cryo-EM structure of human dnmt1 (aa:351-1616) in complex with ubiquitinated paf15 and hemimethylated DNA analog

Reference

Hayashi G, Takahashi Y, Kikuchi A, Kori S, Arita K, Murakami H: "Activation of DNMT1 by Chemically Synthesized Dual monoubiquitinated PAF15 Protein."

Abstract

 

• The contacts include paired nucleotides (mostly a G in Watson-Crick G-C pairing) and amino-acids within a 4.5-Å distance cutoff to base atoms of 5mC.
• The structure is oriented in the base reference frame of 5mC, allowing for easy comparison and direct superimposition between entries.
• The black sphere (•) denotes the 5-methyl carbon atom in 5mC.