SNAP annotations: PDB entry 4v8q

PDB-id

4v8q; DSSR-derived features in text and JSON formats; DNAproDB

Class

ribosome

Method

X-ray (3.1 Å)

Summary

Complex of smpb, a tmrna fragment and ef-tu-gdp-kirromycin with the 70s ribosome

Reference

Neubauer C, Gillet R, Kelley AC, Ramakrishnan V (2012): "Decoding in the absence of a codon by tmRNA and SmpB in the ribosome." Science, 335, 1366-1369. doi: 10.1126/science.1217039.

Abstract

In bacteria, ribosomes stalled at the end of truncated messages are rescued by transfer-messenger RNA (tmRNA), a bifunctional molecule that acts as both a transfer RNA (tRNA) and a messenger RNA (mRNA), and SmpB, a small protein that works in concert with tmRNA. Here, we present the crystal structure of a tmRNA fragment, SmpB and elongation factor Tu bound to the ribosome at 3.2 angstroms resolution. The structure shows how SmpB plays the role of both the anticodon loop of tRNA and portions of mRNA to facilitate decoding in the absence of an mRNA codon in the A site of the ribosome and explains why the tmRNA-SmpB system does not interfere with normal translation.