Summary information and primary citation
- PDB-id
- 1a6y; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (2.3 Å)
- Summary
- Reverba orphan nuclear receptor-DNA complex
- Reference
- Zhao Q, Khorasanizadeh S, Miyoshi Y, Lazar MA, Rastinejad F (1998): "Structural elements of an orphan nuclear receptor-DNA complex." Mol.Cell, 1, 849-861. doi: 10.1016/S1097-2765(00)80084-2.
- Abstract
- The nuclear hormone receptors form the largest known family of transcription factors. The current notion of receptor DNA discrimination, based solely on one major type of hexameric half-site and a highly conserved 66-residue core DNA-binding domain (DBD), does not adequately describe how more than 150 nonsteroid receptors differentiate among response elements. Here, we describe the 2.3 A crystal structure of the DNA-binding region of the orphan receptor RevErb arranged as a tandem homodimer on its optimal response element. The structure reveals the presence of a second major protein-DNA interface adjacent to the classical one involving the half-sites. A sequence comparison of orphan receptors suggests that unique minor-groove interactions involving the receptor hinge regions impart the necessary DNA and dimerization specificity.
