Summary information and primary citation
- PDB-id
- 1bgb; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- hydrolase-DNA
- Method
- X-ray (2.0 Å)
- Summary
- Ecorv endonuclease complex with 5'-cgggatatccc DNA
- Reference
- Horton NC, Perona JJ (1998): "Recognition of flanking DNA sequences by EcoRV endonuclease involves alternative patterns of water-mediated contacts." J.Biol.Chem., 273, 21721-21729. doi: 10.1074/jbc.273.34.21721.
- Abstract
- The 2.1-A cocrystal structure of EcoRV endonuclease bound to 5'-CGGGATATCCC, in a crystal lattice isomorphous with the cocrystallized undecamer 5'-AAAGATATCTT previously determined, shows novel base recognition in the major groove of the DNA flanking the GATATC target site. Lys104 of the enzyme interacts through water molecules with the exocyclic N-4 amino groups of flanking cytosines. Steric exclusion of water molecule-binding sites by the 5-methyl group of thymine drives the adoption of alternative water-mediated contacts with AT versus GC flanks. This structure provides a rare example of structural adaptability in the recognition of different DNA sequences by a protein and suggests preferred strategies for the expansion of target site specificity by EcoRV.
