SNAP output for PDB entry 1bl0 [SNAP web server]

PDB-id

1bl0; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (2.3 Å)

Summary

Multiple antibiotic resistance protein (mara)-DNA complex

Reference

Rhee S, Martin RG, Rosner JL, Davies DR (1998): "A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator." Proc.Natl.Acad.Sci.USA, 95, 10413-10418. doi: 10.1073/pnas.95.18.10413.

Abstract

A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 A thereby bending the DNA by approximately 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.