SNAP output for PDB entry 1d3u [SNAP web server]

PDB-id

1d3u; SNAP-derived features in text and JSON formats; DNAproDB

Class

gene regulation-DNA

Method

X-ray (2.4 Å)

Summary

Tata-binding protein-transcription factor (ii)b-bre+tata-box complex from pyrococcus woesei

Reference

Littlefield O, Korkhin Y, Sigler PB (1999): "The structural basis for the oriented assembly of a TBP/TFB/promoter complex." Proc.Natl.Acad.Sci.USA, 96, 13668-13673. doi: 10.1073/pnas.96.24.13668.

Abstract

Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription.