Summary information and primary citation
- PDB-id
- 1e7k; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- RNA binding protein
- Method
- X-ray (2.9 Å)
- Summary
- Crystal structure of the spliceosomal 15.5kd protein bound to a u4 snrna fragment
- Reference
- Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R (2000): "Crystal Structure of the Spliceosomal 15.5Kd Protein Bound to a U4 Snrna Fragment." Mol.Cell, 6, 1331. doi: 10.1016/S1097-2765(00)00131-3.
- Abstract
- We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
