SNAP output for PDB entry 1g1x [SNAP web server]

PDB-id

1g1x; SNAP-derived features in text and JSON formats; DNAproDB

Class

ribosome

Method

X-ray (2.6 Å)

Summary

Structure of ribosomal proteins s15, s6, s18, and 16s ribosomal RNA

Reference

Agalarov SC, Sridhar Prasad G, Funke PM, Stout CD, Williamson JR (2000): "Structure of the S15,S6,S18-rRNA complex: assembly of the 30S ribosome central domain." Science, 288, 107-112. doi: 10.1126/science.288.5463.107.

Abstract

The crystal structure of a 70-kilodalton ribonucleoprotein complex from the central domain of the Thermus thermophilus 30S ribosomal subunit was solved at 2.6 angstrom resolution. The complex consists of a 104-nucleotide RNA fragment composed of two three-helix junctions that lie at the end of a central helix, and the ribosomal proteins S15, S6, and S18. S15 binds the ribosomal RNA early in the assembly of the 30S ribosomal subunit, stabilizing a conformational reorganization of the two three-helix junctions that creates the RNA fold necessary for subsequent binding of S6 and S18. The structure of the complex demonstrates the central role of S15-induced reorganization of central domain RNA for the subsequent steps of ribosome assembly.