Summary information and primary citation
- PDB-id
- 1i9f; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- de-novo protein-RNA
- Method
- NMR
- Summary
- Structural characterization of the complex of the rev response element RNA with a selected peptide
- Reference
- Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE (2001): "Structural characterization of the complex of the Rev response element RNA with a selected peptide." Chem.Biol., 8, 511-520. doi: 10.1016/S1074-5521(01)00027-8.
- Abstract
- Results: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis.
Conclusions: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state.
