Summary information and primary citation
- PDB-id
- 1iaw; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- hydrolase-DNA
- Method
- X-ray (2.4 Å)
- Summary
- Crystal structure of naei complexed with 17mer DNA
- Reference
- Huai Q, Colandene JD, Topal MD, Ke H (2001): "Structure of NaeI-DNA complex reveals dual-mode DNA recognition and complete dimer rearrangement." Nat.Struct.Biol., 8, 665-669. doi: 10.1038/90366.
- Abstract
- NaeI, a novel DNA endonuclease, shows topoisomerase and recombinase activities when a Lys residue is substituted for Leu 43. The NaeI-DNA structure demonstrates that each of the two domains of NaeI recognizes one molecule of DNA duplex. DNA recognition induces dramatic rearrangements: narrowing the binding site of the Topo domain 16 A to grip DNA, widening that of the Endo domain 8 A to encircle and bend DNA 45 degrees for cleavage, and completely rebuilding the homodimer interface. The NaeI-DNA structure presents the first example of novel recognition of two copies of one DNA sequence by two different amino acid sequences and two different structural motifs in one polypeptide.
