Summary information and primary citation

PDB-id
1io4; SNAP-derived features in text and JSON formats; DNAproDB
Class
transcription-DNA
Method
X-ray (3.0 Å)
Summary
Crystal structure of runx-1-aml1-cbfalpha runt domain-cbfbeta core domain heterodimer and c-ebpbeta bzip homodimer bound to a DNA fragment from the csf-1r promoter
Reference
Tahirov TH, Inoue-Bungo T, Morii H, Fujikawa A, Sasaki M, Kimura K, Shiina M, Sato K, Kumasaka T, Yamamoto M, Ishii S, Ogata K (2001): "Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta." Cell(Cambridge,Mass.), 104, 755-767. doi: 10.1016/S0092-8674(01)00271-9.
Abstract
The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta subunits are essential for differentiation of hematopoietic and bone cells, and their mutation is intimately related to the development of acute leukemias and cleidocranial dysplasia. Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(bZip)-DNA, and AML1/Runx-1/CBFalpha(Runt domain)-DNA complexes. The hydrogen bonding network formed among CBFalpha(Runt domain) and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the allosteric regulation mechanism of CBFalpha(Runt domain)-DNA binding by CBFbeta. The point mutations of CBFalpha related to the aforementioned diseases were also mapped and their effect on DNA binding is discussed.

Cartoon-block schematics in six views (download the tarball)

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