Summary information and primary citation
- PDB-id
- 1jgg; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (2.0 Å)
- Summary
- Even-skipped homeodomain complexed to at-rich DNA
- Reference
- Hirsch JA, Aggarwal AK (1995): "Structure of the even-skipped homeodomain complexed to AT-rich DNA: new perspectives on homeodomain specificity." EMBO J., 14, 6280-6291.
- Abstract
- even-skipped is a homeobox gene important in controlling segment patterning in the embryonic fruit fly. Its homeobox encodes a DNA binding domain which binds with similar affinities to two DNA consensus sequences, one AT-rich, the other GC-rich. We describe a crystallographic analysis of the Even-skipped homeodomain complexed to an AT-rich oligonucleotide at 2.0 A resolution. The structure reveals a novel arrangement of two homeodomains bound to one 10 bp DNA sequence in a tandem fashion. This arrangement suggests a mechanism for the homeoproteins' regulatory specificity. In addition, the functionally important residue Gln50 is observed in multiple conformations making direct and water-mediated hydrogen bonds with the DNA bases.
