Summary information and primary citation

PDB-id
1ksx; SNAP-derived features in text and JSON formats; DNAproDB
Class
replication-DNA
Method
X-ray (3.2 Å)
Summary
Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex
Reference
Enemark EJ, Stenlund A, Joshua-Tor L (2002): "Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex." EMBO J., 21, 1487-1496. doi: 10.1093/emboj/21.6.1487.
Abstract
Initiation of DNA replication of the papillomavirus genome is a multi-step process involving the sequential loading of viral E1 protein subunits onto the origin of replication. Here we have captured structural snapshots of two sequential steps in the assembly process. Initially, an E1 dimer binds to adjacent major grooves on one face of the double helix; a second dimer then binds to another face of the helix. Each E1 monomer has two DNA-binding modules: a DNA-binding loop, which binds to one DNA strand and a DNA-binding helix, which binds to the opposite strand. The nature of DNA binding suggests a mechanism for the transition between double- and single-stranded DNA binding that is implicit in the progression to a functional helicase.

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js