Summary information and primary citation
- PDB-id
- 1m5p; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- translation-RNA
- Method
- X-ray (2.6 Å)
- Summary
- Transition state stabilization by a catalytic RNA
- Reference
- Rupert PB, Massey AP, Sigurdsson ST, Ferre-D'Amare AR (2002): "Transition state stabilization by a catalytic RNA." Science, 298, 1421-1424. doi: 10.1126/science.1076093.
- Abstract
- The hairpin ribozyme catalyzes sequence-specific cleavage of RNA through transesterification of the scissile phosphate. Vanadate has previously been used as a transition state mimic of protein enzymes that catalyze the same reaction. Comparison of the 2.2 angstrom resolution structure of a vanadate-hairpin ribozyme complex with structures of precursor and product complexes reveals a rigid active site that makes more hydrogen bonds to the transition state than to the precursor or product. Because of the paucity of RNA functional groups capable of general acid-base or electrostatic catalysis, transition state stabilization is likely to be an important catalytic strategy for ribozymes.
