Summary information and primary citation
- PDB-id
-
1m5v;
DSSR-derived features in text and
JSON formats; DNAproDB
- Class
- translation-RNA
- Method
- X-ray (2.4 Å)
- Summary
- Transition state stabilization by a catalytic RNA
- Reference
-
Rupert PB, Massey AP, Sigurdsson ST, Ferre-D'Amare AR
(2002): "Transition
state stabilization by a catalytic RNA."
Science, 298, 1421-1424. doi:
10.1126/science.1076093.
- Abstract
- The hairpin ribozyme catalyzes sequence-specific
cleavage of RNA through transesterification of the scissile
phosphate. Vanadate has previously been used as a
transition state mimic of protein enzymes that catalyze the
same reaction. Comparison of the 2.2 angstrom resolution
structure of a vanadate-hairpin ribozyme complex with
structures of precursor and product complexes reveals a
rigid active site that makes more hydrogen bonds to the
transition state than to the precursor or product. Because
of the paucity of RNA functional groups capable of general
acid-base or electrostatic catalysis, transition state
stabilization is likely to be an important catalytic
strategy for ribozymes.
