SNAP output for PDB entry 1m8x [SNAP web server]

PDB-id

1m8x; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA

Method

X-ray (2.2 Å)

Summary

Crystal structure of the pumilio-homology domain from human pumilio1 in complex with nre1-14 RNA

Reference

Wang X, McLachlan J, Zamore PD, Hall TMT (2002): "MODULAR RECOGNITION OF RNA BY A HUMAN PUMILIO-HOMOLOGY DOMAIN." CELL(CAMBRIDGE,MASS.), 110, 501-512. doi: 10.1016/S0092-8674(02)00873-5.

Abstract

Puf proteins are developmental regulators that control mRNA stability and translation by binding sequences in the 3' untranslated regions of their target mRNAs. We have determined the structure of the RNA binding domain of the human Puf protein, Pumilio1, bound to a high-affinity RNA ligand. The RNA binds the concave surface of the molecule, where each of the protein's eight repeats makes contacts with a different RNA base via three amino acid side chains at conserved positions. We have mutated these three side chains in one repeat, thereby altering the sequence specificity of Pumilio1. Thus, the high affinity and specificity of the PUM-HD for RNA is achieved using multiple copies of a simple repeated motif.