SNAP output for PDB entry 1r4i [SNAP web server]

PDB-id

1r4i; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (3.1 Å)

Summary

Crystal structure of androgen receptor DNA-binding domain bound to a direct repeat response element

Reference

Shaffer PL, Jivan A, Dollins DE, Claessens F, Gewirth DT (2004): "Structural basis of androgen receptor binding to selective androgen response elements." Proc.Natl.Acad.Sci.USA, 101, 4758-4763. doi: 10.1073/pnas.0401123101.

Abstract

Steroid receptors bind as dimers to a degenerate set of response elements containing inverted repeats of a hexameric half-site separated by 3 bp of spacer (IR3). Naturally occurring selective androgen response elements have recently been identified that resemble direct repeats of the hexameric half-site (ADR3). The 3D crystal structure of the androgen receptor (AR) DNA-binding domain bound to a selective ADR3 reveals an unexpected head-to-head arrangement of the two protomers rather than the expected head-to-tail arrangement seen in nuclear receptors bound to response elements of similar geometry. Compared with the glucocorticoid receptor, the DNA-binding domain dimer interface of the AR has additional interactions that stabilize the AR dimer and increase the affinity for nonconsensus response elements. This increased interfacial stability compared with the other steroid receptors may account for the selective binding of AR to ADR3 response elements.