SNAP output for PDB entry 1t7p [SNAP web server]

PDB-id

1t7p; SNAP-derived features in text and JSON formats; DNAproDB

Class

transferase-DNA

Method

X-ray (2.2 Å)

Summary

T7 DNA polymerase complexed to DNA primer-template,a nucleoside triphosphate, and its processivity factor thioredoxin

Reference

Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T (1998): "Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution." Nature, 391, 251-258. doi: 10.1038/34593.

Abstract

DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.