Summary information and primary citation
- PDB-id
- 1urn; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription-RNA
- Method
- X-ray (1.92 Å)
- Summary
- U1a mutant-RNA complex + glycerol
- Reference
- Oubridge C, Ito N, Evans PR, Teo CH, Nagai K (1994): "Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin." Nature, 372, 432-438. doi: 10.1038/372432a0.
- Abstract
- The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined at 1.92 A resolution. The ten-nucleotide RNA loop binds to the surface of the beta-sheet as an open structure, and the AUUGCAC sequence of the loop interacts extensively with the conserved RNP1 and RNP2 motifs and the C-terminal extension of the RNP domain. These interactions include stacking of RNA bases with aromatic side chains of proteins and many direct and water-mediated hydrogen bonds. The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain.
