Summary information and primary citation
- PDB-id
- 1vtl; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription-DNA
- Method
- X-ray (2.25 Å)
- Summary
- Co-crystal structure of tbp recognizing the minor groove of a tata element
- Reference
- Kim JL, Nikolov DB, Burley SK (1993): "Co-Crystal Structure of TBP Recognizing the Minor Groove of a TATA Element." Nature, 365, 520-527. doi: 10.1038/365520a0.
- Abstract
- The three-dimensional structure of a TATA-box binding polypeptide complexed with the TATA element of the adenovirus major late promoter has been determined by X-ray crystallography at 2.25 A resolution. Binding of the saddle-shaped protein induces a conformational change in the DNA, inducing sharp kinks at either end of the sequence TATAAAAG. Between the kinks, the right-handed double helix is smoothly curved and partially unwound, presenting a widened minor groove to TBP's concave, antiparallel beta-sheet. Side-chain/base interactions are restricted to the minor groove, and include hydrogen bonds, van der Waals contacts and phenylalanine-base stacking interactions.
