SNAP output for PDB entry 1vto [SNAP web server]

PDB-id

1vto; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (1.9 Å)

Summary

1.9 Å resolution refined structure of tbp recognizing the minor groove of tataaaag

Reference

Kim JL, Burley SK (1994): "1.9 A Resolution Refined Structure of TBP Recognizing the Minor Groove of TATAAAAG." Nat.Struct.Biol., 1, 638-653.

Abstract

The three-dimensional structure of a TATA box-binding protein (TBP) from Arabidopsis thaliana complexed with a fourteen base pair oligonucleotide bearing the Adenovirus major late promoter TATA element has been refined at 1.9 A resolution, giving a final crystallographic R-factor of 19.4%. Binding of the monomeric, saddle-shaped alpha/beta protein induces an unprecedented conformational change in the DNA. A detailed structural and functional analysis of this unusual protein-DNA complex is presented, with particular emphasis on the mechanisms of DNA deformation, TATA element recognition, and preinitiation complex assembly.