Summary information and primary citation
- PDB-id
- 1xsn; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA
- Method
- X-ray (1.95 Å)
- Summary
- Crystal structure of human DNA polymerase lambda in complex with a one nucleotide DNA gap and ddttp
- Reference
- Garcia-Diaz M, Bebenek K, Krahn JM, Kunkel TA, Pedersen LC (2005): "A closed conformation for the Pol lambda catalytic cycle." Nat.Struct.Mol.Biol., 12, 97-98. doi: 10.1038/nsmb876.
- Abstract
- Pol lambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol lambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol lambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.
