Summary information and primary citation

PDB-id
1xsn; SNAP-derived features in text and JSON formats; DNAproDB
Class
transferase-DNA
Method
X-ray (1.95 Å)
Summary
Crystal structure of human DNA polymerase lambda in complex with a one nucleotide DNA gap and ddttp
Reference
Garcia-Diaz M, Bebenek K, Krahn JM, Kunkel TA, Pedersen LC (2005): "A closed conformation for the Pol lambda catalytic cycle." Nat.Struct.Mol.Biol., 12, 97-98. doi: 10.1038/nsmb876.
Abstract
Pol lambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol lambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol lambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.

Cartoon-block schematics in six views (download the tarball)

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