SNAP output for PDB entry 1ytb [SNAP web server]

PDB-id

1ytb; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (1.8 Å)

Summary

Crystal structure of a yeast tbp-tata-box complex

Reference

Kim Y, Geiger JH, Hahn S, Sigler PB (1993): "Crystal structure of a yeast TBP/TATA-box complex." Nature, 365, 512-520. doi: 10.1038/365512a0.

Abstract

The 2.5 A crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.