SNAP output for PDB entry 1zr4 [SNAP web server]

PDB-id

1zr4; SNAP-derived features in text and JSON formats; DNAproDB

Class

recombination-DNA

Method

X-ray (3.4 Å)

Summary

Structure of a synaptic gamma-delta resolvase tetramer covalently linked to two cleaved dnas

Reference

Li W, Kamtekar S, Xiong Y, Sarkis GJ, Grindley ND, Steitz TA (2005): "Structure of a synaptic gamma delta resolvase tetramer covalently linked to two cleaved DNAs." Science, 309, 1210-1215. doi: 10.1126/science.1112064.

Abstract

The structure of a synaptic intermediate of the site-specific recombinase gammadelta resolvase covalently linked through Ser10 to two cleaved duplex DNAs has been determined at 3.4 angstrom resolution. This resolvase, activated for recombination by mutations, forms a tetramer whose structure is substantially changed from that of a presynaptic complex between dimeric resolvase and the cleavage site DNA. Because the two cleaved DNA duplexes that are to be recombined lie on opposite sides of the core tetramer, large movements of both protein and DNA are required to achieve strand exchange. The two dimers linked to the DNAs that are to be recombined are held together by a flat interface. This may allow a 180 degrees rotation of one dimer relative to the other in order to reposition the DNA duplexes for strand exchange.