Summary information and primary citation

PDB-id
2adc; SNAP-derived features in text and JSON formats; DNAproDB
Class
RNA binding protein-RNA
Method
NMR
Summary
Solution structure of polypyrimidine tract binding protein rbd34 complexed with cucucu RNA
Reference
Oberstrass FC, Auweter SD, Erat M, Hargous Y, Henning A, Wenter P, Reymond L, Amir-Ahmady B, Pitsch S, Black DL, Allain FHT (2005): "Structure of PTB bound to RNA: specific binding and implications for splicing regulation." Science, 309, 2054-2057. doi: 10.1126/science.1114066.
Abstract
The polypyrimidine tract binding protein (PTB) is a 58-kilodalton RNA binding protein involved in multiple aspects of messenger RNA metabolism, including the repression of alternative exons. We have determined the solution structures of the four RNA binding domains (RBDs) of PTB, each bound to a CUCUCU oligonucleotide. Each RBD binds RNA with a different binding specificity. RBD3 and RBD4 interact, resulting in an antiparallel orientation of their bound RNAs. Thus, PTB will induce RNA looping when bound to two separated pyrimidine tracts within the same RNA. This leads to structural models for how PTB functions as an alternative-splicing repressor.

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js