Summary information and primary citation
- PDB-id
- 2az2; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- viral protein-RNA
- Method
- X-ray (2.6 Å)
- Summary
- Flock house virus b2-dsrna complex (p4122)
- Reference
- Chao JA, Lee JH, Chapados BR, Debler EW, Schneemann A, Williamson JR (2005): "Dual modes of RNA-silencing suppression by Flock House virus protein B2." Nat.Struct.Mol.Biol., 12, 952-957. doi: 10.1038/nsmb1005.
- Abstract
- As a counter-defense against antiviral RNA silencing during infection, the insect Flock House virus (FHV) expresses the silencing suppressor protein B2. Biochemical experiments show that B2 binds to double-stranded RNA (dsRNA) without regard to length and inhibits cleavage of dsRNA by Dicer in vitro. A cocrystal structure reveals that a B2 dimer forms a four-helix bundle that binds to one face of an A-form RNA duplex independently of sequence. These results suggest that B2 blocks both cleavage of the FHV genome by Dicer and incorporation of FHV small interfering RNAs into the RNA-induced silencing complex.
