Summary information and primary citation
- PDB-id
- 2b2d; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- virus-viral protein-RNA
- Method
- X-ray (2.9 Å)
- Summary
- RNA stemloop operator from bacteriophage qbeta complexed with an n87s,e89k mutant ms2 capsid
- Reference
- Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SEV, Stonehouse NJ, Liljas L, Stockley PG (2006): "Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2." Structure, 14, 487-495. doi: 10.1016/j.str.2005.12.006.
- Abstract
- Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.
