SNAP output for PDB entry 2c62 [SNAP web server]

PDB-id

2c62; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription

Method

X-ray (1.74 Å)

Summary

Crystal structure of the human transcription cofactor pc4 in complex with single-stranded DNA

Reference

Werten S, Moras D (2006): "A Global Transcription Cofactor Bound to Juxtaposed Strands of Unwound DNA." Nat.Struct.Mol.Biol., 13, 181. doi: 10.1038/NSMB1044.

Abstract

The 1.74-A crystal structure of the human transcription cofactor PC4 in complex with a single-stranded 20-mer oligonucleotide reveals how symmetry-related beta-surfaces of the protein homodimer interact with juxtaposed 5-nucleotide DNA regions running in opposite directions. The structure explains high-affinity binding of PC4 to the complementary strands of unwinding duplex DNA, and it suggests the cofactor may have a role in relaxing negative supercoils or exposing unpaired bases for sequence-specific recognition by other biomolecules.