SNAP output for PDB entry 2cjk [SNAP web server]

PDB-id

2cjk; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein

Method

NMR

Summary

Structure of the RNA binding domain of hrp1 in complex with RNA

Reference

Perez-Canadillas JM (2006): "Grabbing the message: structural basis of mRNA 3'UTR recognition by Hrp1." EMBO J., 25, 3167-3178. doi: 10.1038/sj.emboj.7601190.

Abstract

The recognition of specific signals encoded within the 3'-untranslated region of the newly transcribed mRNA triggers the assembly of a multiprotein machine that modifies its 3'-end. Hrp1 recognises one of such signals, the so-called polyadenylation enhancement element (PEE), promoting the recruitment of other polyadenylation factors in yeast. The molecular bases of this interaction are revealed here by the solution structure of a complex between Hrp1 and an oligonucleotide mimicking the PEE. Six consecutive bases (AUAUAU) are specifically recognised by two RNA-binding domains arranged in tandem. Both protein and RNA undergo significant conformational changes upon complex formation with a concomitant large surface burial of RNA bases. Key aspects of RNA specificity can be explained by the presence of intermolecular aromatic-aromatic contacts and hydrogen bonds. Altogether, the Hrp1-PEE structure represents one of the first steps towards understanding of the assembly of the cleavage and polyadenylation machinery at the atomic level.