SNAP output for PDB entry 2ct8 [SNAP web server]

PDB-id

2ct8; SNAP-derived features in text and JSON formats; DNAproDB

Class

ligase-RNA

Method

X-ray (2.7 Å)

Summary

Crystal structure of aquifex aeolicus methionyl-trna synthetase complexed with trna(met) and methionyl-adenylate anologue

Reference

Nakanishi K, Ogiso Y, Nakama T, Fukai S, Nureki O (2005): "Structural basis for anticodon recognition by methionyl-tRNA synthetase." Nat.Struct.Mol.Biol., 12, 931-932. doi: 10.1038/nsmb988.

Abstract

In the 2.7-A resolution crystal structure of methionyl-tRNA synthetase (MetRS) in complex with tRNA(Met) and a methionyl-adenylate analog, the tRNA anticodon loop is distorted to form a triple-base stack comprising C34, A35 and A38. A tryptophan residue stacks on C34 to extend the triple-base stack. In addition, C34 forms Watson-Crick-type hydrogen bonds with Arg357. This structure resolves the longstanding question of how MetRS specifically recognizes tRNA(Met).