Summary information and primary citation

PDB-id
2f8x; SNAP-derived features in text and JSON formats; DNAproDB
Class
transcription-DNA
Method
X-ray (3.25 Å)
Summary
Crystal structure of activated notch, csl and maml on hes-1 promoter DNA sequence
Reference
Nam Y, Sliz P, Song L, Aster JC, Blacklow SC (2006): "Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes." Cell(Cambridge,Mass.), 124, 973-983. doi: 10.1016/j.cell.2005.12.037.
Abstract
Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.

Cartoon-block schematics in six views (download the tarball)

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