SNAP output for PDB entry 2fdc [SNAP web server]

PDB-id

2fdc; SNAP-derived features in text and JSON formats; DNAproDB

Class

DNA binding protein-DNA

Method

X-ray (3.3 Å)

Summary

Structural basis of DNA damage recognition and processing by uvrb: crystal structure of a uvrb-DNA complex

Reference

Truglio JJ, Karakas E, Rhau B, Wang H, DellaVecchia MJ, Van Houten B, Kisker C (2006): "Structural basis for DNA recognition and processing by UvrB." Nat.Struct.Mol.Biol., 13, 360-364. doi: 10.1038/nsmb1072.

Abstract

DNA-damage recognition in the nucleotide excision repair (NER) cascade is a complex process, operating on a wide variety of damages. UvrB is the central component in prokaryotic NER, directly involved in DNA-damage recognition and guiding the DNA through repair synthesis. We report the first structure of a UvrB-double-stranded DNA complex, providing insights into the mechanism by which UvrB binds DNA, leading to formation of the preincision complex. One DNA strand, containing a 3' overhang, threads behind a beta-hairpin motif of UvrB, indicating that this motif inserts between the strands of the double helix, thereby locking down either the damaged or undamaged strand. The nucleotide directly behind the beta-hairpin is flipped out and inserted into a small, highly conserved pocket in UvrB.