Summary information and primary citation
- PDB-id
- 2gjw; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- hydrolase-RNA
- Method
- X-ray (2.85 Å)
- Summary
- RNA recognition and cleavage by an splicing endonuclease
- Reference
- Xue S, Calvin K, Li H (2006): "RNA Recognition and Cleavage by an Splicing Endonuclease." Science, 312, 902-910. doi: 10.1126/science.1126629.
- Abstract
- The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
