Summary information and primary citation
- PDB-id
- 2hdc; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- protein-DNA
- Method
- NMR
- Summary
- Structure of transcription factor genesis-DNA complex
- Reference
- Jin C, Marsden I, Chen X, Liao X (1999): "Dynamic DNA contacts observed in the NMR structure of winged helix protein-DNA complex." J.Mol.Biol., 289, 683-690. doi: 10.1006/jmbi.1999.2819.
- Abstract
- Genesis is an HNF-3/fkh homologous protein. By using multi-dimensional NMR techniques, we have obtained the solution structure and backbone dynamics of Genesis complexed with a 17 base-pair DNA. Our results indicate that both the local folding and dynamic properties of Genesis are perturbed when it binds to the DNA site. Our data show that a conserved flexible amino acid sequence (wing 1) makes dynamic contacts to DNA in the complex and a short helix is induced by Genesis-DNA interactions. Our data indicate that, unlike the HNF-3gamma/DNA complex, a magnesium ion is not required in forming the stable Genesis-DNA complex.
