Summary information and primary citation
- PDB-id
- 2hgh; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription-RNA
- Method
- NMR
- Summary
- Transcription factor iiia zinc fingers 4-6 bound to 5s rrna 55mer (NMR structure)
- Reference
- Lee BM, Xu J, Clarkson BK, Martinez-Yamout MA, Dyson JH, Case DA, Gottesfeld JM, Wright PE (2006): "Induced Fit and 'Lock and Key' Recognition of 5 S RNA by Zinc Fingers of Transcription Factor IIIA." J.Mol.Biol., 357, 275-291. doi: 10.1016/j.jmb.2005.12.010.
- Abstract
- Transcription factor IIIA (TFIIIA) is a Cys2His2 zinc finger protein that regulates expression of the 5 S ribosomal RNA gene by binding specifically to the internal control element. TFIIIA also functions in transport and storage of 5 S RNA by binding directly to the RNA transcript. To obtain insights into the mechanism by which TFIIIA recognizes 5 S RNA, we determined the solution structure of the middle three zinc fingers bound to the central core of 5 S RNA. Finger 4 utilizes "lock and key" recognition to bind in the widened major groove of the pre-structured RNA loop E motif. This interaction is mediated by direct hydrogen bonding interactions with bases. In contrast, recognition of loop A, a flexible junction of three helices, occurs by an induced fit mechanism that involves reorganization of the conserved CAUA motif and structuring of the finger 5-finger 6 interface to form a complementary RNA binding surface.
