Summary information and primary citation
- PDB-id
- 2hyi; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- hydrolase-RNA binding protein-RNA
- Method
- X-ray (2.3 Å)
- Summary
- Structure of the human exon junction complex with a trapped dead-box helicase bound to RNA
- Reference
- Andersen CBF, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H, Andersen GR (2006): "Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA." Science, 313, 1968-1972. doi: 10.1126/science.1131981.
- Abstract
- In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.
