Summary information and primary citation
- PDB-id
- 2i91; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- RNA binding protein-RNA
- Method
- X-ray (2.65 Å)
- Summary
- 60kda ro autoantigen in complex with a fragment of misfolded RNA
- Reference
- Fuchs G, Stein AJ, Fu C, Reinisch KM, Wolin SL (2006): "Structural and biochemical basis for misfolded RNA recognition by the Ro autoantigen." Nat.Struct.Mol.Biol., 13, 1002-1009. doi: 10.1038/nsmb1156.
- Abstract
- The Ro autoantigen is ring-shaped, binds misfolded noncoding RNAs and is proposed to function in quality control. Here we determine how Ro interacts with misfolded RNAs. Binding of Ro to misfolded precursor (pre)-5S ribosomal RNA requires a single-stranded 3' end and helical elements. As mutating most sequences of the helices and tail results in modest decreases in binding, Ro may be able to associate with a range of RNAs. Ro binds several other RNAs that contain single-stranded tails. A crystal structure of Ro bound to a misfolded pre-5S rRNA fragment reveals that the tail inserts into the cavity, while a helix binds on the surface. Most contacts of Ro with the helix are to the backbone. Mutagenesis reveals that Ro has an extensive RNA-binding surface. We propose that Ro uses this surface to scavenge RNAs that fail to bind their specific RNA-binding proteins.
