SNAP output for PDB entry 2mgz [SNAP web server]

PDB-id

2mgz; SNAP-derived features in text and JSON formats; DNAproDB

Class

RNA binding protein-RNA

Method

NMR

Summary

Solution structure of rbfox family asd-1 rrm and sup-12 rrm in ternary complex with RNA

Reference

Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Guntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y (2014): "RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing." Nat.Struct.Mol.Biol., 21, 778-786. doi: 10.1038/nsmb.2870.

Abstract

Tissue-specific alternative pre-mRNA splicing is often cooperatively regulated by multiple splicing factors, but the structural basis of cooperative RNA recognition is poorly understood. In Caenorhabditis elegans, ligand binding specificity of fibroblast growth factor receptors (FGFRs) is determined by mutually exclusive alternative splicing of the sole FGFR gene, egl-15. Here we determined the solution structure of a ternary complex of the RNA-recognition motif (RRM) domains from the RBFOX protein ASD-1, SUP-12 and their target RNA from egl-15. The two RRM domains cooperatively interact with the RNA by sandwiching a G base to form the stable complex. Multichromatic fluorescence splicing reporters confirmed the requirement of the G and the juxtaposition of the respective cis elements for effective splicing regulation in vivo. Moreover, we identified a new target for the heterologous complex through an element search, confirming the functional significance of the intermolecular coordination.