SNAP output for PDB entry 2nll [SNAP web server]

PDB-id

2nll; SNAP-derived features in text and JSON formats; DNAproDB

Class

transcription-DNA

Method

X-ray (1.9 Å)

Summary

Retinoid x receptor-thyroid hormone receptor DNA-binding domain heterodimer bound to thyroid response element DNA

Reference

Rastinejad F, Perlmann T, Evans RM, Sigler PB (1995): "Structural determinants of nuclear receptor assembly on DNA direct repeats." Nature, 375, 203-211. doi: 10.1038/375203a0.

Abstract

Nuclear receptor heterodimers recognize response elements composed of two direct repeats of the consensus sequence 5'-AGGTCA-3' separated by one to five base pairs. The 1.9 A crystal structure of the complex formed by the DNA-binding domains of the 9-cis retinoic acid receptor and thyroid hormone receptor bound to a thyroid-response element shows that the subunits interact through a DNA-supported interface involving the carboxy-terminal extension of the DNA-binding domain of the thyroid hormone receptor. The stereochemistry suggests a mechanism by which heterodimers recognize the inter-half-site spacing between direct repeats.