Summary information and primary citation
- PDB-id
- 2o8k; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transcription-DNA
- Method
- NMR
- Summary
- NMR structure of the sigma-54 rpon domain bound to the-24 promoter element
- Reference
- Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE (2007): "Structural basis of DNA recognition by the alternative sigma-factor, sigma54." J.Mol.Biol., 369, 1070-1078. doi: 10.1016/j.jmb.2007.04.019.
- Abstract
- The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.
