SNAP output for PDB entry 2ww9 [SNAP web server]

PDB-id

2ww9; SNAP-derived features in text and JSON formats; DNAproDB

Class

ribosome

Method

cryo-EM (8.6 Å)

Summary

cryo-EM structure of the active yeast ssh1 complex bound to the yeast 80s ribosome

Reference

Becker T, Bhushan S, Jarasch A, Armache JP, Funes S, Jossinet F, Gumbart J, Mielke T, Berninghausen O, Schulten K, Westhof E, Gilmore R, Mandon E, Beckmann R (2009): "Structure of Monomeric Yeast and Mammalian Sec61 Complexes Interacting with the Translating Ribosome." Science, 326, 1369. doi: 10.1126/SCIENCE.1178535.

Abstract

The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.