SNAP output for PDB entry 2x7n [SNAP web server]

PDB-id

2x7n; SNAP-derived features in text and JSON formats; DNAproDB

Class

ribosomal protein-RNA

Method

cryo-EM (11.8 Å)

Summary

Mechanism of eif6s anti-association activity

Reference

Gartmann M, Blau M, Armache J-P, Mielke T, Topf M, Beckmann R (2010): "Mechanism of Eif6-Mediated Inhibition of Ribosomal Subunit Joining." J.Biol.Chem., 285, 14848. doi: 10.1074/JBC.C109.096057.

Abstract

During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.