Summary information and primary citation
- PDB-id
- 2xca; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA
- Method
- X-ray (2.5 Å)
- Summary
- Ternary complex of sulfolobus solfataricus dpo4 DNA polymerase, 7,8- dihydro-8-oxodeoxyguanine modified DNA and dgtp - magnesium form
- Reference
- Irimia A, Loukachevitch LV, Eoff RL, Guengerich FP, Egli M (2010): "Metal Ion Dependence of the Active Site Conformation of the Trans-Lesion DNA Polymerase Dpo4 from Sulfolobus Solfataricus." Acta Crystallogr.,Sect.F, 66, 1013. doi: 10.1107/S1744309110029374.
- Abstract
- Crystal structures of a binary Mg2+-form Dpo4-DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4-DNA-dCTP/dGTP complexes with 8-oxoG in the template strand have been determined. Comparison of their conformations and active-site geometries with those of the corresponding Ca2+-form complexes revealed that the DNA and polymerase undergo subtle changes as a result of the catalytically more active Mg2+ occupying both the A and B sites.
