Summary information and primary citation

PDB-id
2xca; SNAP-derived features in text and JSON formats; DNAproDB
Class
transferase-DNA
Method
X-ray (2.5 Å)
Summary
Ternary complex of sulfolobus solfataricus dpo4 DNA polymerase, 7,8- dihydro-8-oxodeoxyguanine modified DNA and dgtp - magnesium form
Reference
Irimia A, Loukachevitch LV, Eoff RL, Guengerich FP, Egli M (2010): "Metal Ion Dependence of the Active Site Conformation of the Trans-Lesion DNA Polymerase Dpo4 from Sulfolobus Solfataricus." Acta Crystallogr.,Sect.F, 66, 1013. doi: 10.1107/S1744309110029374.
Abstract
Crystal structures of a binary Mg2+-form Dpo4-DNA complex with 1,N2-etheno-dG in the template strand as well as of ternary Mg2+-form Dpo4-DNA-dCTP/dGTP complexes with 8-oxoG in the template strand have been determined. Comparison of their conformations and active-site geometries with those of the corresponding Ca2+-form complexes revealed that the DNA and polymerase undergo subtle changes as a result of the catalytically more active Mg2+ occupying both the A and B sites.

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