Summary information and primary citation
- PDB-id
- 2xea; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- virus
- Method
- cryo-EM (4.6 Å)
- Summary
- 4.6 angstrom cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300 kev on a 4kx4k ccd camera
- Reference
- Clare DK, Orlova EV (2010): "4.6 A Cryo-Em Reconstruction of Tobacco Mosaic Virus from Images Recorded at 300 Kev on a 4Kx4K Ccd Camera." J.Struct.Biol., 171, 303. doi: 10.1016/J.JSB.2010.06.011.
- Abstract
- Tobacco mosaic virus (TMV) is a plant virus with a highly ordered organisation and has been described in three different structural states: As stacked disks without RNA (X-ray crystallography), as a helical form with RNA (X-ray fibre diffraction) and as a second distinct helical form with RNA (cryo-EM). Here we present a structural analysis of TMV as a test object to assess the quality of cryo-EM images recorded at 300 keV on a CCD camera. The 4.6A TMV structure obtained is consistent with the previous cryo-EM structure and confirms that there is a second helical form of TMV. The structure here also shows that with a similar number of TMV segments an equivalent resolution can be achieved with a 4k CCD camera at 300 keV.
