Summary information and primary citation
- PDB-id
- 2y1j; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA
- Method
- X-ray (2.15 Å)
- Summary
- Crystal structure of a r-diastereomer analogue of the spore photoproduct in complex with fragment DNA polymerase i from bacillus stearothermophilus
- Reference
- Heil K, Kneuttinger AC, Schneider S, Lischke U, Carell T (2011): "Crystal Structures and Repair Studies Reveal the Identity and the Base-Pairing Properties of the Uv-Induced Spore Photoproduct DNA Lesion." Chemistry, 17, 9651. doi: 10.1002/CHEM.201100177.
- Abstract
- UV light is one of the major causes of DNA damage. In spore DNA, due to an unusual packing of the genetic material, a special spore photoproduct lesion (SP lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (Spl), a radical S-adenosylmethionine (SAM) enzyme. We report here the synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone. The oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enzyme as a template to clarify the configuration of the SP lesion and to provide information about the base-pairing properties of the lesion. The structural analysis together with repair studies allowed us to clarify the identity of the preferentially repaired lesion diastereoisomer.
