Summary information and primary citation

PDB-id
3a4k; SNAP-derived features in text and JSON formats; DNAproDB
Class
hydrolase-DNA
Method
X-ray (2.17 Å)
Summary
Crystal structural analysis of hindiii restriction endonuclease in complex with cognate DNA and divalent cations at 2.17 angstrom resolution
Reference
Watanabe N, Takasaki Y, Sato C, Ando S, Tanaka I (2009): "Structures of restriction endonuclease HindIII in complex with its cognate DNA and divalent cations." Acta Crystallogr.,Sect.D, 65, 1326-1333. doi: 10.1107/S0907444909041134.
Abstract
The three-dimensional crystal structures of HindIII bound to its cognate DNA with and without divalent cations were solved at 2.17 and 2.00 A resolution, respectively. HindIII forms a dimer. The structures showed that HindIII belongs to the EcoRI-like (alpha-class) subfamily of type II restriction endonucleases. The cognate DNA-complex structures revealed the specific DNA-recognition mechanism of HindIII by which it recognizes the palindromic sequence A/AGCTT. In the Mg(2+) ion-soaked structure the DNA was cleaved and two ions were bound at each active site, corresponding to the two-metal-ion mechanism.

Cartoon-block schematics in six views (download the tarball)

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