Summary information and primary citation

PDB-id
3bx3; SNAP-derived features in text and JSON formats; DNAproDB
Class
transcription, RNA binding protein-RNA
Method
X-ray (3.0 Å)
Summary
Puf4 t650c-c724r mutant bound to cox17 RNA 3' utr recognition sequence
Reference
Miller MT, Higgin JJ, Hall TMT (2008): "Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p." Nat.Struct.Mol.Biol., 15, 397-402. doi: 10.1038/nsmb.1390.
Abstract
Pumilio/FBF (PUF) family proteins are found in eukaryotic organisms and regulate gene expression post-transcriptionally by binding to sequences in the 3' untranslated region of target transcripts. PUF proteins contain an RNA binding domain that typically comprises eight alpha-helical repeats, each of which recognizes one RNA base. Some PUF proteins, including yeast Puf4p, have altered RNA binding specificity and use their eight repeats to bind to RNA sequences with nine or ten bases. Here we report the crystal structures of Puf4p alone and in complex with a 9-nucleotide (nt) target RNA sequence, revealing that Puf4p accommodates an 'extra' nucleotide by modest adaptations allowing one base to be turned away from the RNA binding surface. Using structural information and sequence comparisons, we created a mutant Puf4p protein that preferentially binds to an 8-nt target RNA sequence over a 9-nt sequence and restores binding of each protein repeat to one RNA base.

Cartoon-block schematics in six views (download the tarball)

PyMOL session file Download PDB file View in 3Dmol.js