SNAP output for PDB entry 3ey1 [SNAP web server]

PDB-id

3ey1; SNAP-derived features in text and JSON formats; DNAproDB

Class

hydrolase-DNA

Method

X-ray (1.6 Å)

Summary

A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution

Reference

Pallan PS, Prakash TP, Li F, Eoff RL, Manoharan M, Egli M (2009): "A conformational transition in the structure of a 2'-thiomethyl-modified DNA visualized at high resolution." Chem.Commun.(Camb.), 15, 2017-2019. doi: 10.1039/b822781k.

Abstract

Crystal structures of A-form and B-form DNA duplexes containing 2'-S-methyl-uridines reveal that the modified residues adopt a RNA-like C3'-endo pucker, illustrating that the replacement of electronegative oxygen at the 2'-carbon of RNA by sulfur does not appear to fundamentally alter the conformational preference of the sugar in the oligonucleotide context and sterics trump stereoelectronics.