Summary information and primary citation
- PDB-id
- 3iay; SNAP-derived features in text and JSON formats;
DNAproDB
- Class
- transferase-DNA
- Method
- X-ray (2.0 Å)
- Summary
- Ternary complex of DNA polymerase delta
- Reference
- Swan MK, Johnson RE, Prakash L, Prakash S, Aggarwal AK (2009): "Structural basis of high-fidelity DNA synthesis by yeast DNA polymerase delta." Nat.Struct.Mol.Biol., 16, 979-986. doi: 10.1038/nsmb.1663.
- Abstract
- DNA polymerase delta (Pol delta) is a high-fidelity polymerase that has a central role in replication from yeast to humans. We present the crystal structure of the catalytic subunit of yeast Pol delta in ternary complex with a template primer and an incoming nucleotide. The structure, determined at 2.0-A resolution, catches the enzyme in the act of replication, revealing how the polymerase and exonuclease domains are juxtaposed relative to each other and how a correct nucleotide is selected and incorporated. The structure also reveals the 'sensing' interactions near the primer terminus, which signal a switch from the polymerizing to the editing mode. Taken together, the structure provides a chemical basis for the bulk of DNA synthesis in eukaryotic cells and a framework for understanding the effects of cancer-causing mutations in Pol delta.
