SNAP output for PDB entry 3j5y [SNAP web server]

PDB-id

3j5y; SNAP-derived features in text and JSON formats; DNAproDB

Class

translation-RNA

Method

cryo-EM (9.7 Å)

Summary

Structure of the mammalian ribosomal pre-termination complex associated with erf1-erf3-gdpnp

Reference

des Georges A, Hashem Y, Unbehaun A, Grassucci RA, Taylor D, Hellen CU, Pestova TV, Frank J (2014): "Structure of the mammalian ribosomal pre-termination complex associated with eRF1*eRF3*GDPNP." Nucleic Acids Res., 42, 3409-3418. doi: 10.1093/nar/gkt1279.

Abstract

Eukaryotic translation termination results from the complex functional interplay between two release factors, eRF1 and eRF3, in which GTP hydrolysis by eRF3 couples codon recognition with peptidyl-tRNA hydrolysis by eRF1. Here, we present a cryo-electron microscopy structure of pre-termination complexes associated with eRF1•eRF3•GDPNP at 9.7 -Å resolution, which corresponds to the initial pre-GTP hydrolysis stage of factor attachment and stop codon recognition. It reveals the ribosomal positions of eRFs and provides insights into the mechanisms of stop codon recognition and triggering of eRF3's GTPase activity.